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psumo1 vector  (Addgene inc)


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    Structured Review

    Addgene inc psumo1 vector
    Psumo1 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/psumo1+vector/pSUMO1+(Plasmid+%2352258)/pmc10404613-235-72-71
    Average 92 stars, based on 10 article reviews
    psumo1 vector - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Sequencing:

    Article Title: Structural insights into the regulation of the human E2∼SUMO conjugate through analysis of its stable mimetic
    Article Snippet: The DNA sequence encoding human UBC9/UBE2I (UniProt P63279 |UBC9_HUMAN) without the initial methionine was cloned from a commercial CpUC19 vector (Sino Biological cat. no. G08N024M70) into a pET28a vector in a way that confers an N-terminal tag MGHHHHHH. .. The protein sequence with a tag is shown below, with residues mutated in this study highlighted in bold: MGHHHHHHSGIALSRLA QER K AWRKDH PFGFVAVPTK NPDGTMNLMN WECAIPGKKG TPWEGGLFKL RMLFKDDYPS SPPKCKFEPP LFHPNV Y PSG TV C LSILEED KDWRPAITIK QILLGIQELL NEPNIQ D P A Q AEAYTIYCQN RVEYEKRVRA QAKKFAPS The expression vector for the full-length human SAE1:SAE2 heterodimer (UniProt sequences Q9UBE0|SAE1_HUMAN and Q9UBT2|SAE2_HUMAN) was obtained by deleting the sequence corresponding to SUMO1 and UBC9 from an Addgene-deposited pSUMO1 vector (plasmid no. 52258) ( ). ..

    Expressing:

    Article Title: Structural insights into the regulation of the human E2∼SUMO conjugate through analysis of its stable mimetic
    Article Snippet: The DNA sequence encoding human UBC9/UBE2I (UniProt P63279 |UBC9_HUMAN) without the initial methionine was cloned from a commercial CpUC19 vector (Sino Biological cat. no. G08N024M70) into a pET28a vector in a way that confers an N-terminal tag MGHHHHHH. .. The protein sequence with a tag is shown below, with residues mutated in this study highlighted in bold: MGHHHHHHSGIALSRLA QER K AWRKDH PFGFVAVPTK NPDGTMNLMN WECAIPGKKG TPWEGGLFKL RMLFKDDYPS SPPKCKFEPP LFHPNV Y PSG TV C LSILEED KDWRPAITIK QILLGIQELL NEPNIQ D P A Q AEAYTIYCQN RVEYEKRVRA QAKKFAPS The expression vector for the full-length human SAE1:SAE2 heterodimer (UniProt sequences Q9UBE0|SAE1_HUMAN and Q9UBT2|SAE2_HUMAN) was obtained by deleting the sequence corresponding to SUMO1 and UBC9 from an Addgene-deposited pSUMO1 vector (plasmid no. 52258) ( ). ..

    Plasmid Preparation:

    Article Title: Structural insights into the regulation of the human E2∼SUMO conjugate through analysis of its stable mimetic
    Article Snippet: The DNA sequence encoding human UBC9/UBE2I (UniProt P63279 |UBC9_HUMAN) without the initial methionine was cloned from a commercial CpUC19 vector (Sino Biological cat. no. G08N024M70) into a pET28a vector in a way that confers an N-terminal tag MGHHHHHH. .. The protein sequence with a tag is shown below, with residues mutated in this study highlighted in bold: MGHHHHHHSGIALSRLA QER K AWRKDH PFGFVAVPTK NPDGTMNLMN WECAIPGKKG TPWEGGLFKL RMLFKDDYPS SPPKCKFEPP LFHPNV Y PSG TV C LSILEED KDWRPAITIK QILLGIQELL NEPNIQ D P A Q AEAYTIYCQN RVEYEKRVRA QAKKFAPS The expression vector for the full-length human SAE1:SAE2 heterodimer (UniProt sequences Q9UBE0|SAE1_HUMAN and Q9UBT2|SAE2_HUMAN) was obtained by deleting the sequence corresponding to SUMO1 and UBC9 from an Addgene-deposited pSUMO1 vector (plasmid no. 52258) ( ). ..



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    92
    Addgene inc psumo1 vector
    Psumo1 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/psumo1+vector/pSUMO1+(Plasmid+%2352258)/pmc10404613-235-72-71
    Average 92 stars, based on 1 article reviews
    psumo1 vector - by Bioz Stars, 2026-09
    92/100 stars
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